CRISPR is a gene editing tool for the treatment of genetic diseases caused by genetic mutations.
A CRISPR system consists of several parts:
1- The DNA on which we want to edit.
2- The RNA guide that we design to identify the desired DNA.
3- The Cas protein that is supposed to cut the identified DNA.
The Crisper system is also used for mutagenesis, gene silencing, sequencing transfer and replacement, gene sequencing binding to regulate gene expression or DNA marking, or to make targeted epigenetic changes.
In CRISPR, gRNA can be designed with purposeful computational methods that can identify the desired location.